Abstract
Multifluorescent, water-dispersible magnesium nanoclusters (Magnozyme) were obtained using a simple and economical synthesis procedure. The prepared particles were 4 nm in size, and they exhibited significant emission at 450, 545, and 628 nm with multiple excitations of 366,469 and 560 nm wavelengths. The prepared particle exhibited a maximum absolute quantum yield of 21.3, 6.8 % and 5 % in red, green and blue spectrum, respectively, with excellent photostability, good ionic strength tolerability, and broad-range pH stability. The prepared Magnozyme demonstrates 95 % cell viability in human glioma brain cell lines (U-87 MG) and can be used as a probe for cellular imaging. Furthermore, imaging with this brain cell revealed significant cytoplasmic accumulation in the red, green, and blue regions. The confocal Z-stack study revealed the presence of Magnozyme at a depth of the cellular level by capturing a series of images at different planer axes (z-axis). Furthermore, In-vivo toxicity assessments and in-vivo imaging in mice revealed the nontoxicity behavior of Magnozyme with their great staining ability in physiological conditions, confirming their candidature toward biological cell imaging/labeling purposes.
Overview
- The study aimed to develop a novel, multifluorescent, water-dispersible magnesium nanocluster (Magnozyme) for biomedical applications.
- The Magnozyme was synthesized using a simple and economical procedure, resulting in 4 nm-sized particles with significant emission at multiple wavelengths.
- The study aimed to evaluate the photophysical properties, cellular viability, and in-vivo toxicity of Magnozyme as a probe for cellular imaging.
Comparative Analysis & Findings
- Magnozyme exhibited excellent photostability, good ionic strength tolerability, and broad-range pH stability, making it an attractive candidate for biological imaging applications.
- The prepared particles demonstrated 95% cell viability in human glioma brain cell lines (U-87 MG) and showed significant cytoplasmic accumulation in the red, green, and blue regions.
- In-vivo imaging in mice revealed the nontoxicity behavior of Magnozyme with their great staining ability in physiological conditions, confirming their candidacy for biological cell imaging/labeling purposes.
Implications and Future Directions
- Magnozyme's multifluorescent properties and excellent biocompatibility make it a promising probe for cellular imaging, potentially leading to breakthroughs in diagnostics and therapy.
- Future studies could investigate the applicability of Magnozyme in other biological systems, such as tumor imaging, stem cell tracking, or inflammatory response monitoring.
- The development of Magnozyme's surface engineering techniques could enhance its stability, circulation time, and targeting capabilities, further expanding its potential applications.