Structural characterization of a pectin-like polysaccharide from Clematis chinensis Osbeck and its anti-rheumatoid arthritis activity.

in International journal of biological macromolecules by Zhijian Wu, Binhua Huang, Dawei Zhang, Qian Yu, Chunyan Yan

TLDR

  • CCPB-2-2, a polysaccharide isolated from C. chinensis, exhibits anti-RA activity by inhibiting cell proliferation, regulating inflammatory responses, and suppressing cell migration.
  • Its putative structure was characterized, and it has great potential as a drug candidate for RA treatment, warranting further research and development.

Abstract

Clematis chinensis Osbeck is a traditional Chinese medicine to treat rheumatic arthritis (RA). We hypothesized that C. chinensis polysaccharide, as a bioactive ingredient, might have the anti-RA function. However, the structure and bioactivity of C. chinensis polysaccharides are less explored. A pectin-like polysaccharide CCPB-2-2 was isolated from C. chinensis root. The putative chemical structure of CCPB-2-2 was characterized through monosaccharide composition, uronic acid reduction, partial acid hydrolysis, methylation reaction, and nuclear magnetic resonance spectroscopy. CCPB-2-2 was composed of the RG-I domain of the pectin moiety and the non-pectin moiety with a backbone with →6)-β-D-Galp-(1→, →3)-β-D-Galp-(1→, →4)-α-D-GlcAp-(1→, →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, and →3,6)-α-D-Manp-(1 → residues. Moreover, the anti-RA activity of CCPB-2-2 was evaluated in MH7A cells. CCPB-2-2 could inhibit lipopolysaccharide -induced MH7A cell proliferation, down-regulate the expression of pro-inflammatory factors (interleukin-6, interleukin-8, and interleukin-1 beta) and matrix metalloproteinase genes (matrix metallopeptidase 1 and matrix metallopeptidase 3), regulate apoptosis proteins (BCL2-associated X and B-cell lymphoma-2) and inhibit cell migration. In conclusion, C. chinensis polysaccharide CCPB-2-2 exhibited great potential as a drug candidate for the treatment of RA, which is worth of further research and development.

Overview

  • The study investigates the anti-rheumatic arthritis (RA) function of Clematis chinensis Osbeck polysaccharide CCPB-2-2.
  • CCPB-2-2 was isolated from the root of C. chinensis and its structure was characterized through various spectroscopic and chemical methods.
  • The primary objective is to evaluate the anti-RA activity of CCPB-2-2 in MH7A cells and explore its potential as a drug candidate for RA treatment.

Comparative Analysis & Findings

  • CCPB-2-2 inhibited lipopolysaccharide-induced MH7A cell proliferation and down-regulated the expression of pro-inflammatory factors (IL-6, IL-8, IL-1 β) and matrix metalloproteinase genes (MMP1 and MMP3).
  • CCPB-2-2 also regulated apoptosis proteins (BAX and BCL2) and inhibited cell migration in MH7A cells.
  • The putative chemical structure of CCPB-2-2 was characterized as a RG-I domain of the pectin moiety and the non-pectin moiety with a specific backbone and residue composition.

Implications and Future Directions

  • CCPB-2-2 has great potential as a drug candidate for RA treatment, warranting further research and development.
  • Future studies should investigate the precise molecular mechanisms underlying the anti-RA activity of CCPB-2-2 and explore ways to optimize its therapeutic efficacy.
  • Additional studies are needed to assess the safety, tolerability, and biocompatibility of CCPB-2-2 in humans and investigate its potential as a complement to conventional RA therapies.
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