Identification of key genes and drug recommendations in diffuse large B-cell lymphoma based on analysis of glutathione-related genes.

in Cytogenetic and genome research by Yu Ren, Aijun He

TLDR

  • The study identified hub genes related to glutathione that are linked to diffuse large B-cell lymphoma and may be used as diagnostic biomarkers or targets for treatment.

Abstract

Various malignancies can be efficiently combated by focusing on glutathione. It is unclear how glutathione-related genes link to diffuse large B-cell lymphoma (DLBCL). Clinical information was gathered from DLBCL patients, and differences in glutathione-related differentially expressed genes (DEGs) between DLBCL and healthy groups were found. Enrichment analysis was run on the DEGs associated with glutathione. We discovered hub genes in glutathione, confirmed hub genes' capacity for diagnosis and function prediction, and estimated drug sensitivity. Immune microenvironmental variations between healthy and DLBCL people were assessed, and hub genes for transcription factor (TF) targeting and miRNAs were found. The glutathione-related DEGs were linked to biological processes such as response to oxidative stress and response to xenobiotic stimulus, according to enrichment analysis. Out of DEGs associated with glutathione, six hub genes were chosen. In the DLBCL population, there was a notable upregulation of the six hub genes. All the genes' AUC values in the diagnostic ability category were more than 0.7, showing strong hub gene diagnostic capacity. The DLBCL population had a high level of T cell infiltration, according to immune infiltration analysis techniques. Similar activities, such as the cell cycle G2/M phase transition and the negative control of organelle formation, are demonstrated by gene function prediction for Hub. According to drug sensitivity prediction, there was a favorable link between KPNA2 with Pracinostat, BRCA1 with B-7100, and LEE-011. The gene KPNA2 was shown to be concurrently targeted by many miRNAs and TFs, according to the miRNA-gene-TF interaction network. The relationship between DLBCL and glutathione-related genes was uncovered by our research, and six glutathione genes were linked to DLBCL. These genes might be used as diagnostic biomarkers or targets for treatment for DLBCL patients.

Overview

  • The study aimed to investigate the link between glutathione-related genes and diffuse large B-cell lymphoma (DLBCL).
  • CLinical data was collected from DLBCL patients and healthy individuals to identify differences in glutathione-related differentially expressed genes (DEGs) between the two groups.
  • The study aimed to identify hub genes in glutathione, predict their function, and estimate their role in drug sensitivity, as well as explore their relationship with the immune microenvironment.

Comparative Analysis & Findings

  • The study found significant differences in glutathione-related DEGs between DLBCL patients and healthy individuals, and hub genes were identified using enrichment analysis.
  • The six hub genes were found to be significantly upregulated in the DLBCL population, and they showed strong diagnostic capacity with AUC values over 0.7.
  • Gene function prediction revealed similar activities among the hub genes, including cell cycle regulation and organelle formation, and drug sensitivity prediction showed a favorable link between some hub genes and specific drugs.

Implications and Future Directions

  • The study's findings suggest that glutathione-related genes may be used as diagnostic biomarkers or targets for treatment for DLBCL patients.
  • Future studies should explore the potential application of the identified hub genes in clinical practice and investigate their relationship with other disease mechanisms.
  • The miRNA-gene-TF interaction network analysis provides a new direction for understanding the regulation of glutathione-related genes and their role in DLBCL.
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