Generation and banking of patient-derived glioblastoma organoid and its application in cancer neuroscience.

in American journal of cancer research by Li Zhou, Jian Yang, Shubei Wang, Pin Guo, Keman Liao, Zhonggang Shi, Jianyi Zhao, Shukai Lin, Ming Yang, Gang Cai, Qing Xia, Jianwei Ge, Jiayi Chen, Yingying Lin

TLDR

  • The study created tiny, 3D models of brain tumors called GBM organoids (GBOs) from patient cells. These GBOs had the same properties as the original tumor. The study also showed how these GBOs could be used to study the brain and develop treatments for GBM. The study's findings have important implications for the treatment of GBM and could lead to personalized medicine for GBM patients.

Abstract

Glioblastoma (GBM) is the most common and deadly tumor in the central nervous system. Although much has been done to optimize treatment options for GBM, the clinical prognosis is still very poor. The recent development of organoid models are emerging as cutting-edge tools in GBM research. However, the established and applications of organoid in cancer neuroscience are still elusive. In this study, we successfully established patient-derived GBM organoids (GBOs) with conserved pathological properties of parental GBM. Moreover, GBO-neuron co-culture system was also investigated and interactions between GFP labeled neurons and mCherry labeled GBOs have been observed. We further used an in-situ stereotaxic instrument to implant GBO into the brains of nude mice and established intracranial orthotopic GBM models based on these GBOs. Thus, we proposed a system to generate and bank patient-derived GBOs and verified its application in cancer neuroscience, which might be an important way to illustrate the mechanism of GBM.

Overview

  • The study focuses on the development of patient-derived GBM organoids (GBOs) with conserved pathological properties of parental GBM. The methodology used for the experiment includes the establishment of GBOs and a GBO-neuron co-culture system. The primary objective of the study is to verify the application of GBOs in cancer neuroscience and illustrate the mechanism of GBM. The study aims to generate and bank patient-derived GBOs and establish intracranial orthotopic GBM models based on these GBOs.

Comparative Analysis & Findings

  • The study compares the outcomes observed under different experimental conditions or interventions, including the establishment of GBOs and a GBO-neuron co-culture system. The results show that GBOs have conserved pathological properties of parental GBM. The study also observes interactions between GFP labeled neurons and mCherry labeled GBOs in the GBO-neuron co-culture system. The key findings of the study are that GBOs can be successfully established and banked, and their application in cancer neuroscience has been verified. The study also illustrates the mechanism of GBM through the establishment of intracranial orthotopic GBM models based on GBOs.

Implications and Future Directions

  • The study's findings have significant implications for the field of research and clinical practice, as they demonstrate the potential of GBOs in cancer neuroscience. The study identifies limitations, such as the need for further validation of GBOs in larger patient populations. Future research directions could include the exploration of the role of GBOs in GBM pathogenesis and the development of targeted therapies for GBM. The study also suggests the potential of GBOs in personalized medicine, where patient-derived GBOs can be used to develop individualized treatments for GBM patients.
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