Characterization and quantification of the interaction between the NFL-TBS.40-63 peptide and lipid nanocapsules.

in International journal of pharmaceutics: X by A Griveau, H Alnemeh-Al Ali, M A Jourdain, A Dupont, J Eyer

TLDR

  • The NFL-peptide and LNCs form a special kind of bracelet that helps them get into the cells better. This new construction is better at getting into the cells than the LNCs alone. The study shows that this new construction could be used to treat glioblastoma.

Abstract

Several studies previously showed that the NFL-TBS.40-63 peptide (NFL-peptide) is capable to specifically penetrating several glioblastoma cell lines (rat, mouse, human) and inhibiting their cell divisionand their tumor development. When lipid nanocapsules (LNCs) are functionalized with the NFL-peptide, their absorption is targeted in glioblastoma cells bothand. In the present study, we investigated the molecular architecture of these nanovectors (LNC-NFL) by using several microscopy techniques (transmission electron microscopy, cryo-electron microscopy, and cryo-electron tomography). We also used high-performance liquid chromatography (UPLC) technique to evaluate the interaction between LNCs and peptides. The work shows that the NFL-peptide forms stable long filaments along which the lipid nanocapsules interact strongly to form some sort of nanomolecular bracelets. This new construction composed of the NFL-peptide and lipid nanocapsules shows a better internalization in rat glioblastoma cells (F98 cells) than lipid nanocapsules alone.

Overview

  • The study investigates the molecular architecture of lipid nanocapsules (LNCs) functionalized with the NFL-peptide in glioblastoma cells. The NFL-peptide forms stable long filaments along which the LNCs interact strongly to form nanomolecular bracelets. The study aims to evaluate the internalization of the NFL-peptide and LNCs in rat glioblastoma cells (F98 cells).
  • Comparative Analysis & Findings
  • The study shows that the NFL-peptide and LNCs form stable long filaments along which the LNCs interact strongly to form nanomolecular bracelets. This new construction composed of the NFL-peptide and LNCs shows a better internalization in rat glioblastoma cells (F98 cells) than LNCs alone.

Comparative Analysis & Findings

  • The NFL-peptide and LNCs form stable long filaments along which the LNCs interact strongly to form nanomolecular bracelets. This new construction composed of the NFL-peptide and LNCs shows a better internalization in rat glioblastoma cells (F98 cells) than LNCs alone.

Implications and Future Directions

  • The study highlights the potential of the NFL-peptide and LNCs as a targeted delivery system for glioblastoma therapy. Future studies should investigate the efficacy of this new construction in animal models of glioblastoma and evaluate its potential for human translation.
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