FromLeaf Galls to Therapeutic Silver Nanoparticles: Antibacterial and Anticancer Applications.

in Pharmaceutics by Ovungal Sabira, Nedumbayil Drisya, Anthyalam Parambil Ajaykumar, Asok Mathew, Kodangattil Narayanan Jayaraj, Valiyaparambil Sivadasan Binitha, Koladath Vasu Zeena, Kanakkassery Balan Roy, Pandikkadan Ayyappan Janish, Padannappurath Sheena, Kaladharan Perumpaparampil Viswanathan

TLDR

  • This study makes silver nanoparticles (AgNPs) using plant extracts. The AgNPs are strong against bacteria, cancer cells, and dividing cells. The study shows that making AgNPs using plant extracts is a good way to make them, and it could be used in medicine or agriculture.

Abstract

The synthesis of silver nanoparticles (AgNPs) using environmentally friendly methods has become increasingly important due to its sustainability and cost-effectiveness. This study investigates the green synthesis of AgNPs using gall extracts from the plant, known for its high phytochemical content. The formation of AgNPs was verified through multiple analytical techniques, including UV-Vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), zeta potential analysis, and dynamic light scattering (DLS). The UV-Vis spectroscopy results displayed a distinct surface plasmon resonance peak indicative of AgNP formation. FTIR analysis revealed specific interactions between silver ions and phytochemicals in the gall extract, while TEM images confirmed the nanoscale morphology and size of the synthesized particles. Zeta potential and DLS analyses provided insights into the stability and size distribution of the AgNPs, demonstrating good colloidal stability. Biological properties of the AgNPs were assessed through various assays. Antimicrobial activity was tested using the disc diffusion method againstand, showing significant inhibitory effects. The anticancer potential was evaluated using the trypan blue exclusion assay on Dalton's Lymphoma Ascites (DLA) cells, revealing considerable cytotoxicity. Additionally, antimitotic activity was studied in the dividing root cells of, where the AgNPs significantly inhibited cell division. This research highlights the effective use ofgall extracts for the green synthesis of AgNPs, presenting an eco-friendly approach to producing nanoparticles with strong antimicrobial, anticancer, and antimitotic properties. The promising results suggest potential applications of these biogenic AgNPs in medical and agricultural sectors, paving the way for further exploration and utilization.

Overview

  • The study investigates the green synthesis of silver nanoparticles (AgNPs) using gall extracts from a plant known for its high phytochemical content. The formation of AgNPs was verified through multiple analytical techniques, including UV-Vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), zeta potential analysis, and dynamic light scattering (DLS). The study aims to demonstrate the eco-friendly approach to producing nanoparticles with strong antimicrobial, anticancer, and antimitotic properties. The primary objective is to provide a sustainable and cost-effective method for the synthesis of AgNPs.

Comparative Analysis & Findings

  • The study compares the outcomes observed under different experimental conditions or interventions detailed in the study. The results of the UV-Vis spectroscopy, FTIR spectroscopy, TEM, zeta potential analysis, and DLS all indicate the successful formation of AgNPs using gall extracts. The antimicrobial activity of the synthesized AgNPs was significant, as shown by the disc diffusion method. The anticancer potential of the AgNPs was evaluated using the trypan blue exclusion assay on Dalton's Lymphoma Ascites (DLA) cells, revealing considerable cytotoxicity. Additionally, the antimitotic activity of the AgNPs was studied in dividing root cells, where the particles significantly inhibited cell division. The key findings of the study demonstrate the effective use of gall extracts for the green synthesis of AgNPs, presenting an eco-friendly approach to producing nanoparticles with strong antimicrobial, anticancer, and antimitotic properties.

Implications and Future Directions

  • The study's findings highlight the potential impact of the synthesized AgNPs on the field of research or clinical practice. The eco-friendly approach to producing nanoparticles with strong antimicrobial, anticancer, and antimitotic properties could lead to the development of new therapeutic agents for various diseases. However, the study has some limitations, such as the need for further characterization of the AgNPs and the evaluation of their toxicity. Future research directions could include the exploration of the optimal conditions for the synthesis of AgNPs using gall extracts, the development of targeted delivery systems for the AgNPs, and the investigation of their potential applications in agriculture and environmental remediation.
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